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KMID : 0380019950100020170
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.170 ~ p.175
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Effect of Ammonium Ion on the Production of a Polysaccharide, Methylan from Methanol by Methylobacterium organophilum
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Oh, Deok Kun/오덕근
Lim, Hyun Soo/Kim, Jung Hoe/임현수/김정희
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Abstract
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The effect of nitrogen source on production of a high viscosity exopolysaccharide, methylan, from methanol by Methylobacterium organophilum was investigated in fed-batch culture. During the fermentation, cells continued to grow even after the nitrogen source added to the medium was depleted and methylan production was stimulated under the condition which ammonium ion was depleted. Cell growth increased proportionally to the initial concentration of ammonium ion in the medium, but methylan production was significantly inhibited at the high concentration of ammonium ion. As the initial concentration of ammonium ion increased, the specific growth rate, the specific product formation rate and the specific substrate consumption rate decreased due to the inhibitory effect of excess ammonium ions. In order to reduce the inhibitory effect by high concentration of ammonium ion. The control of ammonium ion concentration within the desired level(usually 0.45g/P ) was necessary. When ammonium ion concentration was maintained below 0.15g// by exponential feeding, methylan production could be increased up to 12.5g/l.
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